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. 2016 Mar 14;7(17):23837–23849. doi: 10.18632/oncotarget.8070

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Copyright: © 2016 Sagar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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a. Cytoplasmic (cyt) and nuclear (nuc) extracts from HCT116 cells transfected with control (siCNT) or with RPS19-specific siRNA (siS19) were analyzed by western blot with primary antibodies for p53, MDM2, RPS19, Lamin and GAPDH. Quantification from three independent experiments is reported in lower panel as column plot of the mean±s.e.m of the values normalized by control. b. MCF7 cells, transfected with control (siCNT) or with RPS19-specific siRNA (siS19), were fixed and stained with antibodies for p53 and MDM2 and then incubated with FITC–conjugated anti-Rabbit IgG and TRITC-conjugated anti-Moiuse IgG,. The localization of p53 and MDM2 was visualized by fluorescence microscopy.