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. 2016 Mar 19;7(17):23961–23974. doi: 10.18632/oncotarget.8208

Figure 5. Secreted extracellular PAI-1 enhances radiation-induced EMT in radiosensitive NCI-H460 cells.

Figure 5

(A) Effects of PAI-1 on radiation-induced migration in NCI-H460 cells were analyzed by a wound-healing assay. (B) Effects of PAI-1 on morphological changes in NCI-H460 cells were monitored with a 3D culture model. The cells were then permeabilized and stained for tubulin (green) and with DAPI (blue). (C) Effects of PAI-1 on protein expression of E-cadherin, Fibronectin, and Vimentin in NCI-H460 cells were analyzed by Western blotting. (D) Effects of PAI-1 on mRNA expression of CDH1, FN1, and VIM in NCI-H460 cells were analyzed by real time qRT-PCR. *p < 0.05 compared with non-irradiated cells; **p < 0.05 compared with irradiated cells treated with control media; ***p < 0.05 compared with irradiated cells treated with CM. (E) The effects of PAI-1 on protein expression of Snail in NCI-H460 cells were analyzed by Western blotting.