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. 2016 Mar 19;7(17):24005–24017. doi: 10.18632/oncotarget.8209

Figure 1.

Figure 1

(A) Relative expression of stemness associated genes in HepG2 and HepG2.2.15 HCC cells. (B) Flow cytometry dot plot analysis for CD133 expression in HepG2 and HepG2.2.15 HCC cells. (C) Flow cytometry analysis for ALDH activity using the ALDEFLUOR kit in HepG2 and HepG2.2.15 cells. DEAB stands for negative control when cells were treated with an ALDH inhibitor, diethylaminobenzaldehyde. (D) Representative image and quantification of hepatospheres generated from HepG2 and HepG2.2.15 HCC cells. Scale bar = 100 μm. ***p < 0.001. (E) Detection of full-length and C-terminal truncated HBx by RT-PCR using two sets of primers. 44F and XR1 flanks 197 nucleotides. 44F and 465R flanks 465 nucleotides. (F) RT-PCR analysis of full-length and C-terminal truncated HBx in a panel of immortalized normal and HCC cell lines. 18S was amplified as an internal control. CD133 expression was determined by flow cytometry analysis.