Figure 4. Abrogation of negative feedback on YAP in a cell line causes a tumor-associated phenotype.

(A) Colony formation in soft agar was assessed in AML-12 cell lines with the indicated modifications. p-value from two-tailed t-test between results of control cells and Sav1;Lats2 double-knockdown cells is indicated. (B) Molecular changes in core Hippo components caused by Sav1 knockdown and subsequent Lats knockdown. The same cells used in panel (A) were plated and harvested at 90% confluence.