A. Real-time PCR of miR-1229 expression and western blotting of nuclear b-catenin, GSK-3β, APC, and ICAT expression in eight fresh breast cancer tissue specimens (T). U6 was used as the RNA loading control, p84 was used as nuclear control; α-tubulin was used as the protein loading control. B. Correlation between miR-1229 levels and GSK-3β, APC, ICAT, and nuclear β-catenin expression in clinical breast cancer tissues. The expression levels of GSK-3β, APC, ICAT and β-catenin, checked by western blotting analysis, were determined by densitometry. The ratio of second sample (GSK-3β/α-tubulin, APC /α-tubulin, ICAT/α-tubulin and β-catenin /α-tubulin) was considered as 1.0.