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. 2016 Mar 23;7(17):24348–24360. doi: 10.18632/oncotarget.8324

Figure 6. NAC reversed the EMT in HCC cells and the inhibitory effects on invasion and migration of HCC cells were reduced after blocking the EMT.

Figure 6

(A) Western blotting and (B) inverted confocal microscopic images (scale bars, 50 μm) showing the expression of E-cadherin (epithelial marker: green) and vimentin (mesenchymal marker: red) in HCC cells. Nuclei were counterstained with DAPI. (C) Bar graphs based on quantitative data from (A). Data are mean ± SEM. *P < 0.05, **P < 0.01,***P < 0.001 versus control. (D) Wound-closure control, NAC, and NAC plus twist siRNA transfection groups. Representative photographs at 0 and 48 h after creating a wound (magnification 100 ×). (E) Bar graphs based on quantitative data from (D). Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus control. (F) Transwell chambers were used to determine the invasion of cells after twist siRNA transfection in the presence or absence of NAC for 48 h (representative photographs, magnification 100 ×). (G) Bar graphs based on quantitative data from (F). Data are mean ± SEM. *P < 0.05, **P < 0.01,***P < 0.001 versus control. (H) Western hybridization confirmed the effect of twist siRNA transfection. (I) Bar graphs based on quantitative data from (H). Data are mean ± SEM. *P < 0.05, **P < 0.01,***P < 0.001 versus control. Each experiment was repeated at least three times.