Figure 1. HIF-1α expression is stabilized by CoCl2 and DMOG.

A. The expression of HIF-1α increases in SGC-7901 and AGS gastric cancer cell lines, which were treated with the hypoxia mimetic agent CoCl₂ (100μM) from 0h to 48h, and reaches the highest point at 24h. B. Western blot analysis indicates that CoCl₂ makes the expression of HIF-1α more stable in gastric cell lines compared to DMOG. Both cell lines were treated with CoCl₂ and DMOG (300μM) for 24h. C. Western blot analysis indicates that co-transfected Si-HIF-1α (50μM) with CoCl2 could reverse the stabilized effect of CoCl₂ on HIF-1α in both cell lines.