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. 2016 Mar 22;7(17):24572–24584. doi: 10.18632/oncotarget.8249

Figure 4. FADD interference decreased Rheb expression on the transcriptional level.

Figure 4

FADD siRNA was transfected into MCF-7 A. and MDA-MB-231 B. cells for 48 h. FADD mRNA was measured by qRT-PCR normalized to β-actin. C. MCF-7 cells were transfected with 80 μM FADD siRNA/NC for 48 h and then treated with 5 μg/ml Act D for indicated time. Rheb mRNA were quantified by qRT-PCR. D. Luciferase activity of Rheb-promoter reporter was detected in MCF-7 cells transfected with FADD siRNA/NC. Each bar is the mean of at least 3 independent experiments. Data are representedas mean ± S.D. *p < 0.05; **p < 0.01;***p < 0.001.