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. 2016 Mar 22;7(17):24572–24584. doi: 10.18632/oncotarget.8249

Figure 5. FADD regulated mTORC1 activity through Rheb.

Figure 5

MCF-7 A. or MDA-MB-231 B. cells were transfected with FADD siRNA/NC for 48 h. The cell lysates was performed by western blot with indicated antibodies (n=4). C. MCF-7 cells were transfected with FADD siRNA/NC for 24 h and then starved for 24 h. Then cells were stimulated by 20% serum for 15 minutes (n=3). Cells were cotransfected with FADD siRNA/NC with pRK5-Flag-Rheb or control vector for 48 h. Western blot analysis in MCF-7 D. or MDA-MB-231 E. Band intensity was quantified by chemiAnalysi software. Data are represented as mean ± S.D. *p < 0.05; ***p < 0.001. Each bar is the mean of 3 independent experiments.