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. 2016 Mar 25;7(17):24585–24595. doi: 10.18632/oncotarget.8358

Figure 4. Exosomes upregulate autophagic activity and Exo-GF enhances cisplatin-induced autophagy in PC9 cells.

Figure 4

PC9 cells were co-cultured with 10 μg/ml exosomes derived from different disposals for 24 hours, and autophagic activity was investigated by western blot analysis (A1). LC3-II conversion (A2) and P62 degradation (A3) were analyzed using Scion Image software 4.0.3.2. The impact of Exo-GF on cisplatin-induced autophagy was also investigated by western blot analysis (B1) of LC3-II conversion (B2) and P62 degradation (B3) using Scion Image software 4.0.3.2. Data are presented as the mean ± SD (error bar) of at least three independent experiments. *and **represent P < 0.05 and P < 0.01, respectively.