Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1990 Dec;43(12):1014–1018. doi: 10.1136/jcp.43.12.1014

Abnormal fucosylation of ileal mucus in cystic fibrosis: I. A histochemical study using peroxidase labelled lectins.

S Thiru 1, G Devereux 1, A King 1
PMCID: PMC502976  PMID: 2266173

Abstract

Peroxidase conjugated lectins were used to analyse the glycoproteins of small intestinal mucins in normal infants and those with cystic fibrosis to ascertain whether there are any detectable histochemical differences in saccharide composition. A significant decrease in Lotus tetragonolobus (LTG) binding fucose was shown in normal small intestinal mucin starting around 36 weeks' gestation with total absence of staining at term and beyond. In contrast, the age matched patients with cystic fibrosis showed persistent and intense LTG binding of fucose. These results provide the first clear histochemical evidence that cystic fibrosis mucin is abnormal and confirm the findings of previous biochemical studies.

Full text

PDF
1014

Images in this article

1015Image
on p.1015
1015Image
on p.1015
1016Image
on p.1016

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison R. T. Lectins in diagnostic histopathology: a review. Med Lab Sci. 1986 Oct;43(4):369–376. [PubMed] [Google Scholar]
  2. Chace K. V., Leahy D. S., Martin R., Carubelli R., Flux M., Sachdev G. P. Respiratory mucous secretions in patients with cystic fibrosis: relationship between levels of highly sulfated mucin component and severity of the disease. Clin Chim Acta. 1983 Aug 15;132(2):143–155. doi: 10.1016/0009-8981(83)90242-5. [DOI] [PubMed] [Google Scholar]
  3. Clamp J. R., Gough M. Study of the oligosaccharide units from mucus glycoproteins of meconium from normal infants and from cases of cystic fibrosis with meconium ileus. Clin Sci (Lond) 1979 Nov;57(5):445–451. doi: 10.1042/cs0570445. [DOI] [PubMed] [Google Scholar]
  4. Debray H., Decout D., Strecker G., Spik G., Montreuil J. Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins. Eur J Biochem. 1981 Jun;117(1):41–55. doi: 10.1111/j.1432-1033.1981.tb06300.x. [DOI] [PubMed] [Google Scholar]
  5. Di Sant'Agnese P. A., Davis P. B. Research in cystic fibrosis (first of three parts). N Engl J Med. 1976 Aug 26;295(9):481–485. doi: 10.1056/NEJM197608262950905. [DOI] [PubMed] [Google Scholar]
  6. Feizi T. Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens. Nature. 1985 Mar 7;314(6006):53–57. doi: 10.1038/314053a0. [DOI] [PubMed] [Google Scholar]
  7. Frates R. C., Jr, Kaizu T. T., Last J. A. Mucus glycoproteins secreted by respiratory epithelial tissue from cystic fibrosis patients. Pediatr Res. 1983 Jan;17(1):30–34. doi: 10.1203/00006450-198301000-00006. [DOI] [PubMed] [Google Scholar]
  8. Freeman H. J., Lotan R., Kim Y. S. Application of lectins for detection of goblet cell glycoconjugate differences in proximal and distal colon of the rat. Lab Invest. 1980 Apr;42(4):405–412. [PubMed] [Google Scholar]
  9. Holthöfer H., Virtanen I., Kariniemi A. L., Hormia M., Linder E., Miettinen A. Ulex europaeus I lectin as a marker for vascular endothelium in human tissues. Lab Invest. 1982 Jul;47(1):60–66. [PubMed] [Google Scholar]
  10. Hoskins L. C. Human enteric population ecology and degradation of gut mucins. Dig Dis Sci. 1981 Sep;26(9):769–772. doi: 10.1007/BF01309606. [DOI] [PubMed] [Google Scholar]
  11. Jacobs L. R., De Fontes D., Cox K. L. Cytochemical localization of small intestinal glycoconjugates by lectin histochemistry in controls and subjects with cystic fibrosis. Dig Dis Sci. 1983 May;28(5):422–428. doi: 10.1007/BF02430531. [DOI] [PubMed] [Google Scholar]
  12. King A., Mueller R. F., Heeley A. F., Robertson N. R. Diagnosis of cystic fibrosis in premature infants. Pediatr Res. 1986 Jun;20(6):536–541. doi: 10.1203/00006450-198606000-00011. [DOI] [PubMed] [Google Scholar]
  13. Krivan H. C., Roberts D. D., Ginsburg V. Many pulmonary pathogenic bacteria bind specifically to the carbohydrate sequence GalNAc beta 1-4Gal found in some glycolipids. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6157–6161. doi: 10.1073/pnas.85.16.6157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pereira M. E., Kabat E. A. Specificity of purified hemagglutinin (lectin) from Lotus tetragonolobus. Biochemistry. 1974 Jul 16;13(15):3184–3192. doi: 10.1021/bi00712a029. [DOI] [PubMed] [Google Scholar]
  15. Pereira M. E., Kisailus E. C., Gruezo F., Kabat E. A. Immunochemical studies on the combining site of the blood group H-specific lectin 1 from Ulex europeus seeds. Arch Biochem Biophys. 1978 Jan 15;185(1):108–115. doi: 10.1016/0003-9861(78)90149-2. [DOI] [PubMed] [Google Scholar]
  16. Pier G. B. Pulmonary disease associated with Pseudomonas aeruginosa in cystic fibrosis: current status of the host-bacterium interaction. J Infect Dis. 1985 Apr;151(4):575–580. doi: 10.1093/infdis/151.4.575. [DOI] [PubMed] [Google Scholar]
  17. Ryley H. C., Rennie D., Bradley D. M. The composition of a mucus glycoprotein from meconium of cystic fibrosis, healthy pre-term and full-term neonates. Clin Chim Acta. 1983 Nov 30;135(1):49–56. doi: 10.1016/0009-8981(83)90387-x. [DOI] [PubMed] [Google Scholar]
  18. Scanlin T. F., Wang Y. M., Glick M. C. Altered fucosylation of membrane glycoproteins from cystic fibrosis fibroblasts. Pediatr Res. 1985 Apr;19(4):368–374. doi: 10.1203/00006450-198519040-00011. [DOI] [PubMed] [Google Scholar]
  19. Wesley A., Forstner J., Qureshi R., Mantle M., Forstner G. Human intestinal mucin in cystic fibrosis. Pediatr Res. 1983 Jan;17(1):65–69. doi: 10.1203/00006450-198301000-00013. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES