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Target regions for the GA primer (1180 bp) and the Illumina primer (459 bp) showing variable (orange V1‐V9) and conserved (grey) regions in the bacterial 16S rRNA gene (1400 bp) utilised by the two methods. The numbers in V3 to V7 denote the number of GA probes targeting each variable region, in total 54 probes. *Illumina application note; http://res.illumina.com/documents/products/appnotes/appnote_16s_sequencing.pdf. †Position in E. coli (number of base pairs)40
