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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Angiogenesis. 2016 Jun 20;19(4):451–461. doi: 10.1007/s10456-016-9519-4

Fig. 1. Animal models.

Fig. 1

a Model 1: bAVMs were in induced in R26CreER/+;Eng2f/2f mouse line that has a Rose promoter driving and estrogen inducible cre recombinase and an Eng gene with exons 5 and 6 flanked by loxP sites [23] by injection of AAV1-VEGF stereotactically into the basal ganglia to induce brain focal angiogenesis and i.p. injection of 3 doses of tamoxifen (TM) on 3 consecutive days to globally delete the Eng gene. Brain samples were collected 2, 4 and 8 weeks after AAV1-VEGF injection. b Model 2: bAVMs were induced in Alk11f/2f;Ccr2RFP/+/Cx3cr1GFP/+ mice that have Alk1 gene deleted in one allele and floxed in the other allele [25], RFP gene knocked into one allele of Ccr2 gene and GFP knocked into one allele of Cx3cr1 gene [26] through co-injection of Ad-Cre and AAV1-VEGF stereotactically into the cortex to induce brain focal deletion of Alk1 floxed allele and angiogenesis. Brain samples were collected 8 weeks after the vector injection.