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. 2016 Sep 20;11(9):e0161020. doi: 10.1371/journal.pone.0161020

Fig 1. S. pneumoniae flavodoxin absorption and fluorescence spectra.

Fig 1

(a) UV/VIS absorbance of 39 μM holo (solid line) and 134 μM apoflavodoxin (dashed line) in 150 mM NaCl, 100 mM MES, pH 6.5. (b) Spectra related to three redox states of 43 μM SpFld in 150 mM NaCl, 100 mM MES, pH 6.5 at 10°C: Oxidized (solid line), semiquinone (dashed line) with absorption maxima at 592 nm and reduced (dotted line). (c) Fluorescence emission spectra of 20 μM SpFld holoprotein (solid line) and 20 μM apoprotein (dashed line), in 150 mM NaCl, 100 mM MES, pH 6.5, 10°C.