Table 1. Thermal stability parameters for holo and apo SpFld.
Holo Flavodoxina | Apo Flavodoxina | ||||||||
---|---|---|---|---|---|---|---|---|---|
Far-UV CD |
Near-UV CD |
Fluoresc. | Global fitb | DSC | Far-UV CD |
Near-UV CD |
Global fitb | DSC | |
Tm | 44.7±0.4 | 43.7±1 | 44.5±0.3 | 44.6±0.2 | 46.7±0.5 | 30.3±0.1 | 30.4±0.8 | 30.8±2 | 34.2±0.5 |
(°C) | |||||||||
ΔHm | 52±2 | 38±3 | 65.8±0.1 | 51±1 | 61±2 | 51±1 | 50.5±0.6 | 41±5 | 55±2 |
(kcal mol-1) |
a In 150 mM NaCl, 100 mM MES, pH 6.5.
b Parameters obtained through global analysis of the spectroscopic curves using a two-state unfolding model.