Table 2. Apoflavodoxin/FMN binding energetics.

	S. pneumoniaeb	H. pyloria	Anabaena PCC 7119a
Kd (nM)	83±9	27±6	0.26±0.06
ΔG (kcal mol-1)	-9.7±0.1	-10.3±0.1	-13.1±0.1
ΔH0 (kcal mol-1)	-11.6±0.3c	-21.0±0.3	-11.2±0.2
-TΔS (kcal mol-1)	1.9±0.3	10.7±0.3	-1.9±0.2
ΔCp (kcal kmol-1)	-0.5±0.1d	-0.60±0.03	-0.60±0.02
nH	0.1±0.2c	0.1±0.2	0.1±0.2

^a In 50 mM MOPS, pH 7.0 [28]

^b In 100 mM MES, 150 mM NaCl, pH 6.5

^c ΔH₀ and n_H were estimated by linear regression (see Methods).

^d Estimated as the slope of ΔH vs T from experiments at 15, 20 and 25°C