Table 3. Effect of cerulenin on DHA production by recombinant DH5α harboring pColdI-pfaABCD and different pfaE.

Cerulenin treatment (mg/L)	DHA (% of total fatty acid)	DHA content (mg/g DCW)	Biomass (g/L)	DHA yield (mg/L)
pColdI-pfaABCD+pSTV28::pfaE(DHA)
0	3.3±0.1	1.7±0.1	1.1±0.0	1.9±0.1
0.5	4.7±0.2	2.0±0.1	1.0±0.0	2.0±0.1
1	5.2±0.3	2.7±0.2	0.9±0.1	2.4±0.1
2	7.0±0.3	3.4±0.1	0.7±0.1	2.4±0.1
pColdI-pfaABCD+pSTV28::pfaE(DHA-M)
0	1.7±0.1	0.9±0.1	1.2±0.0	1.1±0.1
0.5	2.6±0.2	1.1±0.2	1.1±0.0	1.2±0.2
1	3.2±0.3	1.5±0.2	1.0±0.0	1.5±0.2
2	4.7±0.1	2.1±0.1	0.8±0.0	1.7±0.1
pColdI-pfaABCD+pSTV28::pfaE(EPA)
0	3.7±0.0	1.3±0.1	1.0±0.1	1.3±0.1
0.5	4.2±0.4	1.8±0.2	0.8±0.1	1.4±0.1
1	6.0±0.1	2.5±0.0	0.6±0.0	1.5±0.0
2	4.5±0.2	2.0±0.1	0.6±0.0	1.2±0.1
pColdI-pfaABCD+pSTV28::pfaE(ARA)
0	1.9±0.2	1.1±0.0	1.2±0.1	1.3±0.1
0.5	3.2±0.2	1.7±0.3	1.0±0.0	1.8±0.3
1	2.3±0.2	1.34±0.2	1.0±0.1	1.4±0.1
2	2.1±0.1	1.2±0.1	0.8±0.0	1.0±0.1

Cells were cultured at 15°C with shaking at 180 rpm. The quantification of DHA from each sample was repeated in triplicate (n = 3).