Table 1. PCR and pyrosecquencing primer.

NR3C1 SNPs	Forward Primer	Reverse Primer	Pyrosequencing Primer	TA PCR	Amplicon lenght
ER23/23EK	Bio 5’-GAAGAAAACCCCAGCAGTGTG -3’	5’-GACGCAGAAACCTTCACAGTAGCT -3’	5’-GGTTTTATAGAAGTCCATCA-3’	61°C	98bp
N363S	5’- ACAGCAGGATCAGAAGCCTATTT -3’	Bio 5’-AGTTCAGAGTCCCCAGAGAAGTC -3’	5’- CCGTTGGTTCCGAAA -3’	57°C	119bp
BclI	5’- AGGTCTTGCTCACAGGGTTCTTG -3’	Bio 5’- GAACTTGCAGGAACATTTGAACG -3’	5’- AAGTAGACAAGTTATGTCTG -3’	58°C	128bp

Bio: the 5’ biotin modification of the PCR primer is required for the DNA single strand DNA capture in the Pyrosequencing assay protocol.

TA: annealing temperature for Polimerase Chain Reaction