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. Author manuscript; available in PMC: 2016 Sep 20.
Published in final edited form as: Methods Cell Biol. 2015 May 27;129:251–277. doi: 10.1016/bs.mcb.2015.03.012

Figure 5. Workflow of an array based reverse Y2H screen to generate and identify mutations that disrupt protein-protein interactions.

Figure 5

Refer to sections indicated on the figure for details describing each step. Red X's represent random point mutations introduced by mutagenic PCR. Yeast colonies carrying mutations that disrupt the protein-protein interaction are circled in red.