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. Author manuscript; available in PMC: 2016 Sep 20.
Published in final edited form as: Chem Res Toxicol. 2016 Aug 3;29(8):1355–1364. doi: 10.1021/acs.chemrestox.6b00193

Figure 3. Effect of MAPK inhibitors on the production of 5-LO products in resident peritoneal macrophages.

Figure 3

RPM were pre-incubated with the MAPK inhibitors (10 µM), SB202190 (SB; p38 MAPK inhibitor), U0126 (U; MEK/ERK inhibitor), and SP600125 (SP; JNK inhibitor), or vehicle control for 30 min and then stimulated with A) 16:0/9al-PC (37.5 µM) and B) 16:0/9al-PC (37.5 µM) and zymosan (25 particles/cell) for 1h. After incubation, the 5-LO-derived eicosanoids (LTC4, 6-trans-LTB4, LTB4, and 5-HETE) were analyzed by LC-MS/MS and quantified using standard isotope dilution. Results shown are averages ± SEM (n=3) from three independent experiments. * p< 0.05; ** p<0.01; *** p<0.0001.