Table 1.

Quantitation of eicosanoid production in murine resident peritoneal macrophages (RPM) and alveolar macrophages (AM) after the addition of oxidized lipid (37.5 µM) for 3 min followed by the addition of zymosan for 1h at 37°C. Results shown are averages ± SEM (n=3).

	Total 5-LO products# (ng/106 cells)		Total COX products## (ng/106 cells)
	no zymosan	with zymosan	no zymosan	with zymosan
RPM
no lipid	<0.03	7.35±0.84	0.03±0.01	2.49±0.33
POPC	<0.03ns	7.16±0.62ns	0.03±0.01ns	2.36±0.25ns
16:0/9al-PC	0.29±0.03***	19.4±2**	0.06±0.01ns	0.63±0.08***
hBALF	<0.03	2.92±0.34	0.04±0.01ns	1.32±0.12
16:0/9al-PC + hBALF	0.15±0.03**	5.78±0.51**	0.05±0.02ns	0.41±0.06***
16:0/9COOH-PC	0.11±0.01**	11.4±1.1***	0.06±0.02ns	1.27±0.06**
16:0/5al-PC	0.20±0.02***	14.5±1.3***	0.05±0.02ns	0.73±0.07**
16:0/5COOH-PC	0.08±0.01**	6.79±0.44***	0.04±0.01ns	1.32±0.09**
ozPOPG	0.21±0.04***	17.6±1.9***	0.05±0.02ns	1.02±0.14**
AM
no lipid	<0.03	1.27±0.11	<0.03	0.14±0.03
POPC	<0.03ns	1.19±0.08ns	<0.03ns	0.16±0.03ns
16:0/9al-PC	0.25±0.04**	2.78±0.14**	<0.03ns	0.07±0.01**

^#total 5-LO products – 6-trans-LTB₄, LTB₄, LTC₄, and 5-HETE

^##total COX products – PGE₂ and TXB₂

hBALF – human bronchoalveolar lavage fluid

^nsp>0.05,

^*p<0.05;

^**p<0.01;

^***p<0.0001 compared to the appropriate no lipid control in same column