Skip to main content
. 2016 Sep 20;11(9):e0161882. doi: 10.1371/journal.pone.0161882

Fig 3. Accumulation of Pho1;2 sense and antisense transcripts under contrasting phosphate conditions.

Fig 3

(A) Schematic of Pho1;2 genes indicating the position of primers (solid triangles; see Table 1). (B) Amplification of fragments corresponding to the mature sense-RNAs of ZmPho1;2a, ZmPho1;2b and SbPho1;2 from oligo-dT primed cDNA prepared from roots and shoot of 10-day-old seedlings, fertilized with Hoagland solution adjusted to 1mM (+P) or 0mM (-P) inorganic phosphate. Primer pairs used for amplification indicated by letter codes on the right-hand side of panel. (C) Amplification of fragments corresponding to putative anti-sense transcripts encoded by ZmPho1;2a, ZmPho1;2b from cDNA as Panel A. Primary PCR (left) and nested PCR performed from 1:100,000 dilution of primary reaction (right). Amplification of ZmUBQ and SbUBQ fragments and amplification from genomic DNA template (G) were used as controls. Primer pairs indicated as in (B).