Fig 4. Ac mutagenesis of ZmPho1;2a.

(A) Genetic strategy to mobilize the donor element bti31094::Ac. Individuals homozygous for the donor element, displaying the characteristic 3 Ac dosage pattern of excision from r1-sc:m3 in the triploid aleurone, were crossed as females by T43. A small proportion (~2%) of the progeny kernels were finely spotted, indicating high Ac dose as a result of replicative transposition, and were screened for potential re-insertion into the target gene. (B) Two high Ac dose kernels (white arrows) highlighted among the largely 2 Ac dose progeny of a typical test cross ear.