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. 2016 Sep 20;11(9):e0161882. doi: 10.1371/journal.pone.0161882

Fig 5. PCR screening for novel Ac insertion in ZmPho1;2a.

Fig 5

(A) Schematic of Zmpho1;2a-m1 allele. Reaction 1 (588bp expected product) and reaction 2 (2496bp) are indicated. (B) PCR reactions using gene and Ac specific primers to amplify potential products from DNA pools. Pool with a star shows amplification in complementary reactions 1 and 2. (C) PCR reactions using individual DNA. Amplification from a positive individual in complementary reactions 1 and 2 indicated with a star.