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. 2016 Sep 20;11(9):e0161882. doi: 10.1371/journal.pone.0161882

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© 2016 Salazar-Vidal et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — Recovery of Ac excision events from Zmpho1;2a-m1. (A) Schematic of primer position and fragment lengths corresponding to Zmpho1;2a-m1 (m1) and derived excision events (m1.1). (B) Amplification of apparent wild-type (Wt) and flanking products (5’, 3’) from three different individuals genotypically homozygous Zmpho1;2a-m1 on the basis of kernel phenotype and pedigree. (C) Generation of a BseYI cutting site, not present in wild-type (W22), as the result of an insertion of 8bp following excision of Ac from Zmpho1;2a-m1. (D) BseYI digestion of products amplified in (B) from three genotypically Zmpho1;2a-m1 homozygous individuals and a wild-type (W22) individual. (E) BseYI digestion of products amplified with the insertion spanning primers MS124/MS052 from a wild-type individual (W22) and an individual homozygous for the stable germinal excision allele pho1;2a’m1.1. 1Kb plus DNA ladder (Invitrogen) is loaded on the first lane in B,C and E.