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. 2016 Sep 20;11(9):e0161882. doi: 10.1371/journal.pone.0161882

Table 1. Primers used in this study for CDS amplification and transcript accumulation.

Target ID Primer 5’-3’ Description
Pho1;2a A MS103- TCGCGGAGGATGGTGAAGTTCT Full CDS (2463bp)
H MS062- GTGTGTCCATTCCTGGAACTCT
F MS002- AGGTGGCCATGAAGTACCTG Exon 6,7,8,9 and 10 (734bp/1130bp)
G MS056- CCTGCATTGCTCTCCAGTAGTAA
Pho1;2b I MS013- ATCCCACGATGGTGAAGTTCT Full CDS (2263bp)
N MS077- ACAATTCTCAATCGACCACTAGC
L RS138- AACTCCGTCTCGGTATGGTGGAGTCT Exon 13, 14 and 3’UTR (383bp/475bp)
M RS139- TGACCAGAACGCCTCATGTTATACCC
SbPho1;2 Q MS105- CATGGACTGGGGCTTCTTAAAC Exon 13, 14 and 15(293bp/475bp)
R MS106- GTAATGGGACAGTCTTCACTGCT
cis-NATPho1;2a B MS121- GAGCATCCTGATTCCATATCTACC Flanking putative exon (478bp)
E MS120- CCCGTAATGGAAGCTTTTACTG
cis-NATPho1;2b J MS131- CGTACTGCTGATCGCATGCATA Flanking putative exon (544bp)
K MS132- AGTACGTGATCAGTGATCTACACTC
cis-NATSbPho1;2 O MS162- CTGATCGCTGACAGATGGCCATA Flanking putative exon (475bp)
P MS158- ACCAGCATCCAGCACCCAAAC
Nested cis-NAT C MS155- GGAGGTGATGGCGGCGCTGGA Putative exon (263bp(Pho1;2a/b),
D MS163- TCTCGGCGTGCTGGACCTTCTT 266bp(SbPho1;2))
ZmUBQ/SbUBQ FWD- CTACAACATTCAGAAGGAGAGCAC
REV- TCTGCAAGGGTACGGCCATCC
ZmCDK FWD- GGAAGGTATGCACAGGACAGAT
REV- TTCAGCACAATCTTGGCAAAAC