Figure 3.

Effects of combined treatment with ultraviolet radiation (UVA and UVB) on the mitochondrial membrane potential (MMP). (A) Rho°206 and (B) 143B cells were seeded at a concentration of 1×10⁶ cells/ml, and then exposed different doses of UV irradiation (Table I). After three washes, the cells were incubated at 37°C for 30 min with 10 µM rhodamine 123 (Rh123) in PBS. The fluorescence was measured using flow cytometry (excitation wavelengths were both 488 nm, and emission wavelengths were both 525 nm). The test was repeated three times and the image presented is representative of these three independent tests. Each bar represents the means ± SD from three experiments. (A and B) There were signficant changes in MMP in both cell lines at different doses of UV radiation. (C) A comparison between 143B and Rho°206 cell lines shows that there was no significant difference in MMP between the SIG groups (P>0.05). However, there were significant differences between the cell lines at the same irradiation doses.