Skip to main content
. 2016 Aug 11;38(4):1047–1054. doi: 10.3892/ijmm.2016.2704

Figure 3.

Figure 3

5-Azacytidine inhibits EC9706 cell metastasis via the upregulation of cadherin 1 (CDH1). CDH1 siRNA attenuated the inhibitory effects of 5-azacytidine on the metastasis of EC9706 cells. (A) Western blot analysis was used to detect the expression of CDH1 in EC9706 cells. Compared with the negative control (DMSO-treated cells), the expression of CDH1 was increased following treatment with 5-azacytidine (**P<0.01), whereas the expression of CDH1 was significantly reduced following CDH1-targeted siRNA transfection (*P<0.05). (B) Transwell assay was used to detect the invasion of EC9706 cells. EC9706 cell invasion was significantly inhibited by 5-azacytidine (**P<0.01), but not by DMSO, whereas the invasive ability of the cells was increased by CDH1 siRNA transfection (**P<0.01). The panels correspond to the following groups: top left panel, DMSO-treated vehicle control; top right panel, 5-azacytidine-treated cells; middle left panel, cells treated with 5-azacytidine and transfected with the control siRNA; middle right panel, cells treated with 5-azacytidine and transfected with CDH1 siRNA; bottom panel, untreated cells. (C) Western blot analysis was used to detect matrix metalloproteinase (MMP)2 and MMP9 expression. The results revealed that the expression of MMP2 and MMP9 was significantly reduced following treatment with 5-azacytidine in the EC9706 cells (**P<0.01 and *P<0.05), while the expression of MMP2 and MMP9 was increased by CDH1 siRNA transfection (**P<0.01 and *P<0.05). 5-aza, 5-azacytidine.