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. 2016 Aug 18;38(4):1055–1062. doi: 10.3892/ijmm.2016.2708

Figure 1.

Figure 1

Figure 1

Figure 1

Knockdown of xanthine oxidase (XO) attenuates the hypoxia/reoxygenation (H/R)-induced production of ROS. (A) Representative western blot of XO and tubulin expression in cardiac microvascular endothelial cells (CMECs) transfected with either no siRNA, negative control siRNA or xanthine dehydrogenase (XDH) siRNA. (B) Representative data for flow cytometric analysis of DCFH-DA-stained CMECs transfected with negative control siRNA or XDH siRNA, and compared with the H/R group. (C) Summary data (n=3 biological replicates) of flow cytometric analysis of DCFH-DA-stained CMECs under conditions of H/R transfected with negative control siRNA or XDH siRNA, and compared with the H/R group. **p<0.01 vs. H/R group.