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. 2016 Aug 11;38(4):1063–1074. doi: 10.3892/ijmm.2016.2706

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Copyright: © Mateu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Closure of defect by keratinocytes. (A) Neonatal keratinocytes (NKs, newborn) closed the standard defect in culture at the stage of confluent growth faster than (B) adult keratinocytes (AKs, adult). (C) Numerous small NKs positive for keratin 8 (K8; green signal) form a thick border closing the defect after 24 h. The border of the defect is very tight and the number of K8-positive cells is highly limited in the case of AKs. The cells were stained with high molecular weight keratin (red signal) and K8. Scale bar is 8 mm (A and B) and 100 μm (C).