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. 2016 Aug 11;38(4):1063–1074. doi: 10.3892/ijmm.2016.2706

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Copyright: © Mateu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Influence of the age of fibroblasts on the phenotype of keratinocytes. (A) Adult fibroblasts (AFs) had a negative effect on the incidence of small neonatal keratinocytes (NKs) located on peripheries of compact colonies positive for keratin 8 (K8; green signal), keratin 19 (K19; red signal) and keratin 14 (K14; red signal). HMW represents high molecular weight cytokeratin and K wide spectrum cytokeratin. The nuclei of the cells were visualized with DAPI (blue color). (B) Neonatal fibroblasts (NFs) had a strong stimulatory effect on the occurrence of small adult keratinocytes (AKs) positive for the above-mentioned keratins. Scale bar is 100 μm. (C) Obtained phenotype of keratinocytes is summarized schematically; blue color represents lacking of the markers of low maturation (small size, K8, K19) and so high differentiation status of keratinocytes with this phenotype. Increasing intensity of red hue correlates with the higher incidence of these markers and so with decreasing maturation of keratinocytes.