Fig. 3.

(a) FLIM images of capillary tubes filled with POPOP, NADH and Rhodamine 6G, submerged in a scattering tissue phantom. As expected POPOP has maximum peak in channel 1, NADH in channel 2 and Rhodamine 6G in channel 3. Rhodamine 6G emitted when excited with both 355 nm or 532 nm. The average lifetimes derived from the time-resolved images where: POPOP (1.26 ± 0.04ns), NADH (0.42 ± 0.03 ns) and Rhodamine (3.51 ± 0.13 ns and 3.59 ± 0.06 ns when excited with 355 and 532nm respectively). (b) OCT volume (2.4 x 2.4 x 0.98 mm) image of the submersed capillary tubes overlaid with the combined lifetime data from channel 2 and channel 3.