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. Author manuscript; available in PMC: 2016 Sep 20.
Published in final edited form as: Immunity. 2016 Jan 5;44(1):73–87. doi: 10.1016/j.immuni.2015.11.011

Figure 7. Btk Is Involved in Integrin-Mediated Outside-In Signaling and FcRγ-Mediated Functions.

Figure 7

(A–C) Superoxide release of WT and Btk−/− neutrophils stimulated with 3 μM fMLF (A), plated on a polyvalent integrin ligand-coated surface (pRGD) without stimulus (B) or with 3 μM fMLF (C). Control values were subtracted from stimulated values.

(D) Quantitative binding of fibrinogen-coated fluorescent beads to unstimulated WT and Btk−/− neutrophils, stimulated with 1 μM fMLF, 10 μg/ml IgG immune complexes, or a combination of both as determined by flow cytometry.

(E) Phagocytosis of IgG-coated fluorescent beads by WT and Btk−/− neutrophils incubated at 37°C with or without 1 μM fMLF for indicated time points.

(F) Respective statistics of phagocytosis after 1 hr.

Depicted are mean + SEM of n ≥ 3 independent performed experiments; *p < 0.05; **p < 0.01; ***p < 0.001. See also Figure S7.