Figure 2. The ASP depends on the components of the PCP system in the wing disc and not in the ASP.
ASPs marked by membrane-tethered GFP and expressing RNAi constructs in either the ASP (driven by btl-Gal4) or the dorsal compartment of the wing disc (driven by ap-Gal4) and directed against the indicated genes. Genotypes: (A) btl-Gal4 UAS-CD8:GFP/+; UAS-pkRNAi/+; (B) btl-Gal4 UAS-CD8:GFP/UAS-VangRNAi; (C) btl-Gal4 UAS-CD8:GFP/+; UAS-gugRNAi/+; (D) btl-Gal4 UAS-CD8:GFP/UAS-dallyRNAi; UAS-dallyRNAi/+; (E) btl-Gal4 UAS-CD8:GFP/+; UAS-dlpRNAi/+; (F) ap-Gal4/+; btl-LHG lexO-CD2:GFP/UAS-pkRNAi; (G) ap-Gal4/UAS-VangRNAi; btl-LHG lexO-CD2:GFP/+; (H) ap-Gal4/+; UAS-gugRNAi/btl-LHG lexO-CD2:GFP; (I) ap-Gal4/UAS-dallyRNAi; btl-LHG lexO-CD2:GFP/UAS-dallyRNAi; (J) ap-Gal4/+; btl-LHG lexO-CD2:GFP/UAS-dlpRNAi. Scale bar: 25 µm.
Figure 2—figure supplement 1. Dependence of ASP cytonemes on Prickle and Van Gogh expression.
Over-expression of pk or Vang in the ASP (btl-Gal4 UAS-pk and btl-Gal4 UAS-Vang) had no effect on the number of ASP cytonemes (p value from student t test 0.51 and 0.42, respectively, compared to control) or on ASP morphology. Over-expression in the disc (ap-Gal4 UAS-pk and ap-Gal4 UAS-Vang) decreased the number of ASP cytonemes (p value from student t test 0.0022 and 0.0020, respectively, compared to control, and 0.0046 and 0.0012 compared to btl-Gal4 UAS-pk and btl-Gal4 UAS-Vang, respectively) and affected ASP morphology. Over-expression of fz and fmi in either the ASP or disc was without apparent consequence (p values from student t tests 0.20–0.9).
Figure 2—figure supplement 1—source data 1. Numbers of ASP cytonemes in flies over-expressing the PCP components.
elife-18979-fig2-figsupp1-data1.xlsx (38.3KB, xlsx)
DOI: 10.7554/eLife.18979.011