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. 2016 Sep 3;5:e18979. doi: 10.7554/eLife.18979

Figure 9. Genetic interaction between laminin mutants and integrin mutants.

Figure 9.

(A,E,I) RNAi targeting expression of the laminin gene wb in the wing disc -altered ASP morphology and reduced ASP cytonemes (A) and reduced Dpp (dad-GFP fluorescence; E) and FGF signaling (α-dpERK staining; I). Genotypes: (A,I) ap-Gal4/+; btl-LHG lexO-CD2:GFP/UAS-wbRNAi; (E) ap-Gal4/+;dad-GFP/UAS-wbRNAi. (B–D, F–H, J–L) ASP morphology and number of ASP cytonemes (B,C), Dpp signaling (F,G) and FGF signaling (J,K) was normal in mew and wb heterozygotes but not in mew wb double heterozygotes (D,H,L). Arrows indicate signaling cells and dashed white lines indicate ASPs. Genotypes: (B) mewM6/+; btl-LHG lexO-Cherry-CAAX/+; (C) wb3/+; btl-LHG lexO-Cherry-CAAX/+; (D) mewM6/+; wb3/+; btl-LHG,lexO-Cherry-CAAX/+; (F) mewM6/+; dad-GFP/+; (G) wb3/+; dad-GFP/+; (H) mewM6/+; wb3/+; dad-GFP/+; (J) mewM6/+; (K) wb3/+; (L) mewM6/+; wb3/+. (J–L) Staining was with α-Dlg and α-dpERK antibodies. Scale bar: 25 µm.

DOI: http://dx.doi.org/10.7554/eLife.18979.026

Figure 9—source data 1. Figure 9 data - Cytoneme and fluorescence quantification.
(A) The number of ASP cytonemes of ap>wbRNAi flies. (B–D) The numbers of ASP cytonemes in mew/+, wb/+ and mew/+; wb/+ flies. (E,I) Quantification of Dad-GFP fluorescence and dpERK staining in ap>wbRNAi flies. (F–H) Quantification of Dad-GFP fluorescence in mew/+, wb/+ and mew/+; wb/+ flies. (J–L) Quantification of dpERK staining in mew/+, wb/+ and mew/+; wb/+ flies.
DOI: http://dx.doi.org/10.7554/eLife.18979.027
elife-18979-fig9-data1.xlsx (36.9KB, xlsx)
DOI: 10.7554/eLife.18979.027