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. 2016 Aug 5;283(18):3371–3388. doi: 10.1111/febs.13811

Figure 4.

Figure 4

ENPP1 is able to hydrolyse protein poly(ADP‐ribosyl)ation. About 70 nm of human recombinant PARP1 was automodified to produce ~ 3 μm PAR substrate (defined in monomeric ADP‐ribose units) and incubated with buffer only (control) and decreasing concentrations of mENPP2‐1‐T (A), mENPP2‐1‐8xHis (B) and NUDT16 (C). Samples were fractionated on SDS/PAGE and transferred on nitrocellulose membranes. Membranes were first stained with S‐Ponceau and then probed with anti‐PAR antibody. (D) Time point hydrolysis of PARylated PARP1 was performed at indicated concentrations and times with NUDT16, mENPP2‐1‐T and SVP. Samples were resolved on SDS/PAGE and transferred on nitrocellulose membranes. Membranes were first stained with S‐Ponceau and then probed with anti‐PAR antibody.