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. Author manuscript; available in PMC: 2017 Sep 30.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Aug 31;478(4):1617–1623. doi: 10.1016/j.bbrc.2016.08.169

Fig. 4.

Fig. 4

A. Immunopurified antiserum was tested on MCF-7 cells ± hGBP-1. After immunopurification, the polyclonal antisera against hGBP-1 only recognized cells expressing hGBP-1. B. TMX2-28 cells were stained for hGBP-1, TUBB3, and PIM1 and analyzed for intracellular distribution by confocal microscopy at 1 µm resolution. These cells express PIM-1L. C. OVCAR 420 cells were analyzed by triple label indirect IF for hGBP-1, TUBB3, and PIM-1 by confocal microscopy with 1 µm sections. OVCAR420 cells express PIM-1L. D. A blow-up of the OVCAR420 cells shows that vesicles containing hGBP-1 associate with microtubules.