FIGURE 3.
16S rRNA-based phylogenetic tree of moonmilk cultivable isolates (MM). The collection points, from which each moonmilk isolate originates are indicated by colored symbols, while the phylotype affiliations are indicated in brackets. The Streptomyces isolates originating from moonmilk deposits of the Bolshaya Oreshnaya Cave in Siberia are marked by a star. The alignment of nearly complete 16S rRNA sequences had 1413 unambiguously aligned nucleotide positions for 113 strains. The evolutionary model was GTR+I+Γ4 and bootstrap values are based on 100 pseudoreplicates (the bootstrap values below 40% are not displayed). Saccharopolyspora erythraea was used as outgroup, and its branch was reduced five times which is indicated by the slanted bars. The scale bar represents 0.05 substitutions per site.