Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In vitro cleavage activity of RepB6 on ssDNA oligos at different temperatures. A 23-mer oligo substrate (100 nM), radioactively labeled in 5′, was incubated with RepB6 at three different temperatures (30, 37, and 60°C) in the presence of 20 mM of MnCl2 and at the indicated protein:oligo substrate molar ratios. The products were separated on 20% PAA, 8M urea denaturing gels (upper part). Nicking activity of RepB6 was quantified as the percentage of 15-mer product formed (lower part).
