Figure 6.

ITC analysis of Mn²⁺ binding to RepB₆, OBD, and OBD^D42A. Symbols represent the heat released by mole of Mn²⁺ injected as a function the Mn²⁺/protein molar ratio measured at 25°C in ITC buffer. Titrations were performed by adding 1 mM MnCl₂ to RepB₆(A), OBD (B), or OBD^D42A (C) proteins at concentrations ranging from 95 to 119 μM. The binding parameters derived from the fit of the single site binding model to the experimental curve are shown at the bottom and the corresponding theoretical curves are depicted as solid lines.