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The strains harboring the GFP fusions to PnpA and RNase J1 show a wild type phenotype. Light microscopy of strains (A) 168 (WT), GP1698 (pnpA-gfp), and GP1748 (ΔpnpA) and (B) 168 (WT), GP1722 (rnjA-gfp), and GP2502 (ΔrnjA). The strains were grown in LB medium at 37°C to stationary phase. Both strains harboring the GFP fusions form short cells comparable to the wild type strain and are not elongated as their respective deletion mutants. Scale bar, 5 μm. WT, wild type.
