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. 2016 Sep 21;7:1492. doi: 10.3389/fmicb.2016.01492

FIGURE 3.

FIGURE 3

The strains harboring the GFP fusions to CshA, PfkA, and Enolase do not show growth defects. (A) Strains 168 (WT), GP1721 (cshA-gfp), and GP1035 (ΔcshA) were streaked on LB agar and incubated at 28°C overnight. The strain harboring the cshA-gfp fusion in the genome is able to grow under these conditions while the deletion mutant has a growth defect. (B) Strains 168 (WT), GP1720 (pfkA-gfp), and GP1747 (ΔpfkA) were streaked on CE minimal medium plates with 0.5% glucose and incubated for 48 h at 37°C. The strain with the pfkA-gfp fusion is able to grow under these conditions, in contrast to the deletion mutant. (C) Strains 168 (WT), GP1700 (eno-gfp), and GP594 (Δeno) were streaked on LB plates and incubated at 37°C overnight. The strain harboring the eno-gfp fusion can grow under these conditions, while the deletion mutant is unable to grow.