FIGURE 5.

The RNA-degrading exoribonuclease PnpA, as well as the glycolytic enzymes enolase and PfkA are localized to the cytoplasm, where they appear uniformly distributed. Fluorescence microscopy of strains harboring GFP fusions. (A) The strains GP1698 (pnpA-gfp) was grown in LB medium at 28°C to mid-exponential phase. The GFP fusion protein can is localized to the cytoplasm, where it appears homogeneously distributed. (B) Strain GP1700 (eno-gfp) was grown to mid-exponential phase in LB medium at 28°C or 37°C. The protein appears widespread in the cytoplasm at both temperatures but at 37°C some cells show bright spots localized at the poles. (C) The strain GP1720 (pfkA-gfp) was grown in LB medium at 28°C to mid-exponential phase and the fusion protein is evenly distributed within the cytoplasm. Scale bar, 2 μm. PC, phase contrast.