Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 20;7(5):e01530-16. doi: 10.1128/mBio.01530-16

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 White et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 3 — Identification of protein binding sites on HPV16 E7 and on PTPN14. (A) Schematic of HPV16 and PTPN14 domain organization and mutants used in the study (39). (B) N/Tert-1 cells expressing wild-type (wt) HPV16 E7-Flag-HA, one of several mutants of HPV16 (E7), or the empty vector were subjected to immunoprecipitation with HA antibody. Immunoprecipitates were separated by SDS-PAGE and Western blotted using antibodies to HA, PTPN14, UBR4, RB1, and actin. (Left panels) Protein levels in input lysates. (Right panels) Proteins recovered after immunoprecipitation with anti-HA antibody. (C) N/Tert-1 cells expressing wild-type HPV16 E7-Flag-HA were transduced with retroviruses expressing V5-tagged PTPN14 or one of several deletion mutant forms of PTPN14 and were harvested 48 h posttransduction. Cell lysates were subjected to immunoprecipitation with HA antibody. Immunoprecipitates were separated by SDS-PAGE and Western blotted using antibodies to HA, V5, and actin. (Top panels) Protein levels in input lysates. (Bottom panel) V5-tagged PTPN14 recovered after immunoprecipitation with anti-HA antibody.