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. 2016 Sep 20;7(5):e01530-16. doi: 10.1128/mBio.01530-16

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Copyright © 2016 White et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Overexpression of the PTPN14 PTP domain can block the interaction of E7 with full-length PTPN14 and restore PTPN14 levels. (A) N/Tert-1 cells expressing wild-type (wt) HPV16 E7-Flag-HA or the empty vector were mock transduced, transduced with a retrovirus packaged with the empty vector, or transduced with retroviruses expressing wild-type V5-tagged PTPN14, the PTPN14 L2 domain, or the PTPN14 PTP domain and were harvested 48 h posttransduction. Steady-state levels of full-length PTPN14, V5-tagged PTPN14 fragments, HA-tagged E7, and actin were measured by Western blotting. (B) N/Tert-1 cells expressing wild-type HPV18 E7-Flag-HA were transduced with retroviruses expressing wild-type V5-tagged PTPN14, the PTPN14 L2 domain, the PTPN14 PTP domain, or the empty vector and were harvested 48 h posttransduction. Cell lysates were subjected to immunoprecipitation with HA antibody. Immunoprecipitates were separated by SDS-PAGE and Western blotted using antibodies to PTPN14, HA, V5, and actin. (Left panels) Protein levels in input lysates. (Right panels) Proteins recovered after immunoprecipitation with anti-HA antibody. HC, antibody heavy chain.