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. 2016 Sep 20;7(5):e01530-16. doi: 10.1128/mBio.01530-16

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Copyright © 2016 White et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — Papillomavirus E7 proteins vary in their ability to bind PTPN14 and target PTPN14 for degradation. (A) N/Tert-1 cells expressing one of several HPV E7 proteins or BPV1 E7 protein tagged with Flag and HA were subjected to immunoprecipitation with HA antibody. Immunoprecipitates were separated by SDS-PAGE and Western blotted using antibodies to HA, PTPN14, UBR4, RB1, and actin. (Left panels) Protein levels in input lysates. (Right panels) Proteins recovered after immunoprecipitation with anti-HA antibody. PTPN14 and HA protein amounts were quantified using ImageJ. The ratio of PTPN14 protein amount relative to E7-HA protein amount in the E7-expressing cell lines is indicated. (B) PTPN14 and actin protein levels in C127 and ID13 cell lysates were measured by Western blotting.