Table 3.
Scavenging effects of 1–5 on different in vitro assays.
| Assay | Scavenging effects (IC50 µg/mL) | |||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | Reference compounds | |
| DPPH radical | 39.39 ± 2.57 | 83.43 ± 5.11 | 12.49 ± 3.98 | 10.21 ± 4.23 | 15.45 ± 7.51 | Ascorbic acid 54.14 ± 2.87 Gallic acid 3.76 ± 0.82 |
| NO radical | 48.69 ± 6.27 | 85.04 ± 4.83 | 30.29 ± 3.48 | 25.47 ± 4.70 | 31.52 ± 2.93 | Ascorbic acid 31.27 ± 5.21 Quercetin 17.30 ± 1.98 |
| O2 •− | 8.87 ± 1.05 | 18.86 ± 2.19 | 6.03 ± 0.39 | 5.76 ± 0.73 | 7.21 ± 3.22 | Ascorbic acid 5.11 ± 0.48 |
| Hydroxyl radical | 44.13 ± 4.28 | 62.34 ± 3.78 | 37.45 ± 2.58 | 33.17 ± 6.83 | 36.52 ± 5.40 | Ascorbic acid 30.29 ± 6.17 |
| Hydrogen peroxide | 91.81 ± 4.57 | 143.13 ± 7.55 | 80.73 ± 6.76 | 87.68 ± 9.43 | 101.03 ± 10.88 | Ascorbic acid 443.76 ± 7.66 |
|
| ||||||
| Scavenging effects (%) | ||||||
|
| ||||||
| Chelating activity 50 µg/mL |
76 | 43 | 89 | 84 | 85 | Ascorbic acid 91 |
| Peroxidationof BSA | Ascorbic acid 94 |
|||||
| 100 µg/mL | 30 | 25 | 36 | 38 | 32 | |
| 200 µg/mL | 55 | 48 | 60 | 64 | 59 | |
| 300 µg/mL | 83 | 65 | 90 | 97 | 92 | |
|
| ||||||
| TEAC (nM) | ||||||
|
| ||||||
| TEAC: ABTS | 28.11 ± 2.48 | 49.51 ± 4.15 | 19.38 ± 3.29 | 16.23 ± 2.15 | 20.34 ± 2.17 | Curcumin 12.04 ± 4.19 |
The data represent the means ± SD of three determinations. IC50: concentration required to inhibit 50% of the activity; DPPH: 2,2-diphenyl-1-picrylhydrazyl; O2 •−: superoxide radical; TEAC: ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging capacity in Trolox equivalence (nanomoles); BSA: bovine serum albumin; Trolox: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.