Figure 6.

NXT/PPARα signaling inhibits cell autophagy. (a) H9c2 cells were treated with 200 μM H₂O₂ as indicated time. Cell lysates were subjected to Western blot. (b) H9c2 cells were treated with or without 0.5 μg/mL NXT for 12 h. After that, cells were treated with 200 μM H₂O₂ for 6 h. Cell lysates were subjected to Western blot. (c) H9c2 cells were transfected with control shRNA or PPARα shRNA for 24 h. Cells were pretreated with or without 0.5 μg/mL NXT for 12 h, and then cells were treated with 200 μM H₂O₂ for 6 h. Cell lysates were subjected to Western blot. Cells were treated with 50 μM bafilomycin A for 1 h before cell lysis. Data are triplicates from three independent experiments.