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. 2016 Sep 21;6:33702. doi: 10.1038/srep33702

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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During recordings of partial vesicle release in the cell bleb model, three types of amperometric current spikes were observed after cholesterol incubations: (a) spike without post spike foot, (b) spike with “ramp” shaped post spike foot, and (c) spike with “ramp+plateau” shaped post spike foot. (d) The probability of observing different types of spikes is presented as a function of cholesterol incubation time at cells (n = 5). Data are expressed as average ± square error mean (SEM). ***p < 0.001 using a two-tailed unpaired student’s t-test.