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. 2016 Sep 21;6:33785. doi: 10.1038/srep33785

Figure 4. Further deletion analysis of the BnSP11-1 promoter.

Figure 4

(A) Promoter deletion constructs (P7-GUS, P8-GUS, P9-GUS, P10-GUS and P11-GUS) and representative GUS staining results. Numbers indicate positions relative to the BnSP11-1 translation start site. Photographs were taken after 16 h GUS incubation. Relative expression of each construct in stamen is represented by +(positive) or −(negative). ‘Lines’ indicate numbers of individual transformants displaying stamen GUS activity over total number of transformants analyzed; Bars = 2 mm. (B) Representative GUS staining results for semithin sections from stages 9 to 12 in transgenic lines of P7, P8, P9, P10 and P11. T, tapetum; Msp, microspore; PG, pollen grain; Bars = 50 μm. (C) Summary of BnSP11-1 promoter deletion analysis results. By sequence alignment of the deleted promoter fragments, a palindromic sequence (TAACTAGTTA, red box) and a putative cis-element (TTCTA, green box) responsible for the spatial and temporal expression patterns of BnSP11-1 were identified.